Biochemical perturbations of the mitotic spindle in Xenopus extracts using a diffusion-based microfluidic assay
نویسندگان
چکیده
منابع مشابه
Biochemical perturbations of the mitotic spindle in Xenopus extracts using a diffusion-based microfluidic assay.
A microfluidic device is a powerful tool to manipulate in a controlled manner at spatiotemporal scales for biological systems. Here, we describe a simple diffusion-based assay to generate and measure the effect of biochemical perturbations within the cytoplasm of cell-free extracts from Xenopus eggs. Our approach comprises a microliter reservoir and a model cytoplasm that are separated by a syn...
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The African clawed frog Xenopus laevis has been instrumental to investigations of both development and cell biology, but the utility of this model organism for genetic and proteomic studies is limited by its long generation time and unsequenced pseudotetraploid genome. Xenopus tropicalis, which is a small, faster-breeding relative of X. laevis, has recently been adopted for research in developm...
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ژورنال
عنوان ژورنال: Biomicrofluidics
سال: 2015
ISSN: 1932-1058
DOI: 10.1063/1.4926324